Coding

Part:BBa_J100100

Designed by: Erin McGuire   Group: Campbell M Lab   (2012-10-05)


E. coli optimized CDM

E. coli optimized codon encoding the caffeine demethylase developed by Christina Smolke lab. Converts caffeine into theophylline.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 3184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 3185
    Illegal PstI site found at 3199
    Illegal NotI site found at 7
    Illegal NotI site found at 3192
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 146
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 3185
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 3185
    Illegal PstI site found at 3199
    Illegal AgeI site found at 105
    Illegal AgeI site found at 766
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23


[edit]
Categories
Parameters
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